The Cetrahe R&D unit (R&D Unit for Expertise and Transfer in Tracing Applied to Hydrogeology and the Environment of the University of Orléans, France) was asked to design and perform a validation programme to evaluate the sensitivity and measurement accuracy of Hyperion fluorometers manufactured by Valeport Water against Cetrahe’s spectrofluorometer (Hitachi Spectrofluorometer F-2500) by carrying out fluorescence measurements for different concentrations of Uranine (Fluorescein) and Sulforhodamine B.
In order to carry out measurements with both the fluorometer and the spectrofluorometer, a simple methodology was drawn up.
Solutions with different and unknown concentrations were made and the concentrations were measured with both the Spectrofluorometer and the Hyperion. The results can be seen in the graph below.
Conclusion of the laboratory evaluation
An extract from the report written by Cetrahe: “The raw results show consistent values between the measurements obtained by the fluorometer and the laboratory’s spectrofluorometer. […] The first series of tests showed a very good sensitivity of the “Valeport” fluorometer with regard to the detection of the two tracers and a very honourable measurement performance.”
These laboratory tests with Cetrahe demonstrated good measurement quality and accuracy of Valeport Water’s Hyperion fluorometer compared to a reference spectrometer.
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